Pierce Bca Assay: A Comprehensive Guide To Protein Quantification
The Pierce Bicinchoninic Acid (BCA) Assay is a widely used method for protein quantification. It hinges on the binding of Cu2+ ions to protein molecules, forming a colored complex with bicinchoninic acid (BCA). The intensity of this complex, measured spectrophotometrically, is proportional to the protein concentration. The assay reagents include BCA solution, protein standard, and …
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